ELECTRON MICROSCOPIC LOCALIZATION OF MITOCHONDRIAL ADENOSINE TRIPHOSPHATASE ACTIVITY
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چکیده
منابع مشابه
Studies on the cornea. V. Electron microscopic localization of adenosine triphosphatase activity in the rabbit cornea in relation to transport
The distribution of ATPase activity in adult and 7-day-old rabbit corneas has been studied by electron microscopic-histochemical techniques. In the cornea! endothelium, the lead phosphate end product of the ATPase reaction is localized at the lateral margins of the cells and in the intercellular spaces. This activity is first demonstrable at 7 days. Pinocytotic vesicles, even those clearly cont...
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The epididymides of rat testis were fixed in glutaraldehyde and cut as frozen sections. The sections were incubated in lead nitrate solution containing as a substrate either ATP, AMP, creatinine phosphate, beta glycerophosphate, or phenyl phosphate. Then they were postfixed in osmium tetroxide, embedded, sectioned, and examined with the electron microscope. In the sperm tail, when ATP is used a...
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Triphenylsulphonium ions inhibit mitochondrial oxidative phosphorylation and adenosine triphosphatase activity. The site of action is on the soluble F1 adenosine triphosphatase component. Triphenylsulphonium ions also inhibit electron transfer in the NAD-cytochrome b region of the respiratory chain. In both types of inhibition, triphenylsulphonium ions are effective at low concentrations, half-...
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Previous studies reported from this and other Laboratories have shown that isolated mitochondria are able to maintain concentration gradients of Na+ and K+ ions. When incubated in media containing an oxidizable substrate and ATP, mitochondria from liver (Macfarlane & Spencer, 1953; Spector, 1953; Price, Fonnesu & Davies, 1956), kidney (Bartley & Davies, 1954; Ulrich, 1961), heart and skeletal m...
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The localization of ATPase(1) activity has been studied by light and electron microscopy in the epidermis of Rana pipiens, Rana catesbiana, and Bufo marinus. The reaction was carried out on skin (glutaraldehyde-fixed or fresh) sectioned with or without freezing. Best results were obtained with nonfrozen sections of fixed tissue. The incubation mixture was either a Wachstein-Meisel medium, or a ...
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1968
ISSN: 0022-1554,1551-5044
DOI: 10.1177/16.10.645